1. Xuǎnzé héshì de yí yè qì yí qǔ biāozhǔn róngyè (rúshuǐ, huǎnchōng yè, xīshì de yán róngyè hé suān jiǎn róngyè) shí duō shǐyòng kōngqì zhìhuàn yí yè qì, yí qǔ jùyǒu gāo huīfā xìng, gāo niánchóu dù yǐjí mìdù dàyú 2.0G/cm de yètǐ huòzhě zài línchuáng jùhé méi liàn fǎnyìng (PCR) cèdìng zhōng de jiā yàng shí shǐyòng zhèng xiàng zhìhuàn yí yè qì. Rú yí qǔ 15ul de yètǐ, zuì hǎo xuǎnzé zuìdà liàngchéng wèi 20ul de yí yè qì, xuǎnzé 50ul jí qí yǐshàng liàngchéng de yí yè qì dōu bùgòu zhǔnquè. 2. Shè dìng yí yètǐjī tiáojié yí yè qì de yí yètǐjī kòngzhì xuánniǔ jìnxíng yí yè liàng de shè dìng. Tiáojié yí yè liàng shí, yīng shì tǐjī dàxiǎo ér xuánzhuǎn kèdù zhì chāoguò shè dìng tǐjī de kèdù, zài huítiáo zhì shè dìng tǐjī, yǐ bǎozhèng yí qǔ de zuì jiā jīngquè dù. 3. Zhuāngpèi xī tóu shǐyòng dān tōngdào yí yè qì shí, jiāng kě tiáo shì yí yè qì de zuǐ zhuī duì zhǔn xī tóu guǎn kǒu, qīng qīng yònglì chuízhí xià yā shǐ zhī zhuāng jǐn. Shǐyòng duō tōngdào yí yè qì shí, jiāng yí yè qì de dì yī pái duì zhǔn dì yī gè guǎn zuǐ, qīngxié chārù, qiánhòu shāowéi yáodòng níngjǐn. 4. Yí yè bǎozhèng yí yè qì, xī tóu hé dài yí qǔ yì tǐ chǔyú tóngyī wēndù; ránhòu yòng dài yí qǔ xī yètǐ rùn xǐ xī tóu 1-2 cì, yóuqí shì niánchóu de yètǐ huò mìdù yǔ shuǐ bùtóng de yètǐ. Yí qǔ yì tǐ shí, jiāng xī tóu jiānduān chuízhí jìnrù yè miàn yǐxià 2-3mm shēndù (yánjìn jiāng xī tóu quánbù chārù róngyè zhōng), huǎnmàn jūnyún dì sōng kāi cāozuò gān, dài xī tóu xīrù róngyè hòu jìng zhì 2-3 miǎo, bìng xié tiē zài róngqì bì shàng tǎng zǒu xī tóu wàibì duōyú de yètǐ. 5. Yí yè qì dì fàngzhì shǐyòng yí yè qì wánbì hòu, yòng dà mǔzhǐ àn zhù xī tóu tuī gǎn xiàng xià yā, ānquán tuìchū xī tóu hòu jiāng qí róngliàng tiáo dào biāozhì de zuìdà zhí, ránhòu jiāng yí yè qì xuánguà zài zhuānyòng de yí yè qì jià shàng; chángqí bùyòng shí yīng zhì yú zhuānyòng hé nèi [1] .展开575/50001. Choose the right pipette
When using standard solutions (such as water, buffer, diluted salt solution and acid-base solution), use an air displacement pipette to remove liquids with high volatility, high viscosity and density greater than 2.0g/cm or in clinical A positive displacement pipette was used for the loading in the polymerase chain reaction (PCR) assay. For pipetting 15 ul of liquid, it is best to choose a pipette with a maximum range of 20 ul. It is not accurate enough to select a pipette of 50 ul or more.
2. Set the pipetting volume
Adjust the pipetting volume control knob of the pipette to set the pipetting amount. When adjusting the amount of pipetting, the scale should be rotated to the scale exceeding the set volume, and then adjusted back to the set volume to ensure the best accuracy of the removal.
3. Assembly tips
When using a single-channel pipette, align the tip of the adjustable pipette with the tip of the pipette and gently press it down firmly to tighten it. When using a multi-channel pipette, align the first row of the pipette with the first nozzle, insert it obliquely, and tighten it slightly before and after.
4. Pipetting
Ensure that the pipette, tip and liquid to be removed are at the same temperature; then rinse the tip 1-2 times with the liquid to be removed, especially a viscous liquid or a liquid of a different density than water. When removing the liquid, immerse the tip of the tip vertically at a depth of 2-3 mm below the liquid level (do not insert the tip into the solution), slowly and evenly loosen the lever, and let the tip inhale the solution for 2-3 seconds. And slanting on the wall of the container to remove excess liquid from the outer wall of the tip.
5. Placement of the pipette
After using the pipette, press down on the tip pusher with your thumb, safely exit the tip and adjust its capacity to the maximum value of the marker, then hang the pipette on the dedicated pipette holder; It should be placed in a special box when not in use for a long time
